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Pituitary Adenylate Cyclase-activating Polypeptides Directly Stimulate Sympathetic Neuron Neuropeptide Y Release through PAC1 Receptor Isoform Activation of Specific Intracellular Signaling Pathways -- Braas and May 274 (39): 27702 -- Journal of Biological Chemistry HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS QUICK SEARCH: [advanced] Author: Keyword(s): Year: Vol: Page: This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Braas, K. M. Articles by May, V. Search for Related Content PubMed PubMed Citation Articles by Braas, K. M. Articles by May, V. J Biol Chem, Vol. 274, Issue 39, 27702-27710, September 24, 1999 Pituitary Adenylate Cyclase-activating Polypeptides Directly Stimulate Sympathetic Neuron Neuropeptide Y Release through PAC1 Receptor Isoform Activation of Specific Intracellular Signaling Pathways Karen M. Braas and Victor May From the Department of Anatomy and Neurobiology, College of Medicine, University of Vermont, Burlington, Vermont 05405 Pituitary adenylate cyclase-activating polypeptides (PACAP) have potent regulatory and neurotrophic activities on superior cervical ganglion (SCG) sympathetic neurons with pharmacological profiles consistent for the PACAP-selective PAC1 receptor. Multiple PAC1 receptor isoforms are suggested to determine differential peptide potency and receptor coupling to multiple intracellular signaling pathways. The current studies examined rat SCG PAC1 receptor splice variant expression and coupling to intracellular signaling pathways mediating PACAP-stimulated peptide release. PAC1 receptor mRNA was localized in over 90% of SCG neurons, which correlated with the cells expressing receptor protein. The neurons expressed the PAC1(short)HOP1 receptor but not VIP/PACAP-nonselective VPAC1 receptors; low VPAC2 receptor mRNA levels were restricted to ganglionic nonneuronal cells. PACAP27 and PACAP38 potently and efficaciously stimulated both cAMP and inositol phosphate production; inhibition of phospholipase C augmented PACAP-stimulated cAMP production, but inhibition of adenylyl cyclase did not alter stimulated inositol phosphate production. Phospholipase C inhibition blunted neuron peptide release, suggesting that the phosphatidylinositol pathway was a prominent component of the secretory response. These studies demonstrate preferential sympathetic neuron expression of PACAP-selective receptor variants contributing to regulation of autonomic function. Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc. This article has been cited by other articles: T. Mustafa, M. Grimaldi, and L. E. Eiden The Hop Cassette of the PAC1 Receptor Confers Coupling to Ca2+ Elevation Required for Pituitary Adenylate Cyclase-activating Polypeptide-evoked Neurosecretion J. Biol. Chem., March 16, 2007; 282(11): 8079 - 8091. [Abstract] [Full Text] [PDF] K. M. Braas, V. May, P. Zvara, B. Nausch, J. Kliment, J. D. Dunleavy, M. T. Nelson, and M. A. Vizzard Role for pituitary adenylate cyclase activating polypeptide in cystitis-induced plasticity of micturition reflexes Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R951 - R962. [Abstract] [Full Text] [PDF] S. L. Gray, N. Yamaguchi, P. Vencova, and N. M. Sherwood Temperature-Sensitive Phenotype in Mice Lacking Pituitary Adenylate Cyclase-Activating Polypeptide Endocrinology, October 1, 2002; 143(10): 3946 - 3954. [Abstract] [Full Text] [PDF] V. May, M. R. Schiller, B. A. Eipper, and R. E. Mains Kalirin Dbl-Homology Guanine Nucleotide Exchange Factor 1 Domain Initiates New Axon Outgrowths via RhoG-Mediated Mechanisms J. Neurosci., August 15, 2002; 22(16): 6980 - 6990. [Abstract] [Full Text] [PDF] K. Drahushuk, T. D. Connell, and D. Higgins Pituitary Adenylate Cyclase-Activating Polypeptide and Vasoactive Intestinal Peptide Inhibit Dendritic Growth in Cultured Sympathetic Neurons J. Neurosci., August 1, 2002; 22(15): 6560 - 6569. [Abstract] [Full Text] [PDF] M. S. Silveira, M. R. Costa, M. Bozza, and R. Linden Pituitary Adenylyl Cyclase-activating Polypeptide Prevents Induced Cell Death in Retinal Tissue through Activation of Cyclic AMP-dependent Protein Kinase J. Biol. Chem., May 3, 2002; 277(18): 16075 - 16080. [Abstract] [Full Text] [PDF] S. Boehm and H. Kubista Fine Tuning of Sympathetic Transmitter Release via Ionotropic and Metabotropic Presynaptic Receptors Pharmacol. Rev., March 1, 2002; 54(1): 43 - 99. [Abstract] [Full Text] [PDF] D. E. Hansel, V. May, B. A. Eipper, and G. V. Ronnett Pituitary Adenylyl Cyclase-Activating Peptides and {alpha}-Amidation in Olfactory Neurogenesis and Neuronal Survival In Vitro J. Neurosci., July 1, 2001; 21(13): 4625 - 4636. [Abstract] [Full Text] [PDF] A. Nicot and E. DiCicco-Bloom Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression PNAS, April 10, 2001; 98(8): 4758 - 4763. [Abstract] [Full Text] [PDF] K. Pavelock, K. M. Braas, L'H. Ouafik, G. Osol, and V. May Differential Expression and Regulation of the Vascular Endothelial Growth Factor Receptors Neuropilin-1 and Neuropilin-2 in Rat Uterus Endocrinology, February 1, 2001; 142(2): 613 - 622. [Abstract] [Full Text] [PDF] R. E. ZIGMOND Neuropeptide Action in Sympathetic Ganglia: Evidence for Distinct Functions in Intact and Axotomized Ganglia Ann. N.Y. Acad. Sci., December 1, 2000; 921(1): 103 - 108. [Full Text] [PDF] V. MAY, M. M. BEAUDET, R. L. PARSONS, and K. M. BRAAS PACAP Modulates Rat Sympathetic Neuron Depolarization Through IP3 Ann. N.Y. Acad. Sci., December 1, 2000; 921(1): 186 - 194. [Full Text] [PDF] R. L. PARSONS, T. M. ROSSIGNOL, M. A. CALUPCA, J. C. HARDWICK, and K. M. BRAAS PACAP Peptides Modulate Guinea Pig Cardiac Neuron Membrane Excitability and Neuropeptide Expression Ann. N.Y. Acad. Sci., December 1, 2000; 921(1): 202 - 210. [Abstract] [Full Text] [PDF] M. M. Beaudet, R. L. Parsons, K. M. Braas, and V. May Mechanisms Mediating Pituitary Adenylate Cyclase-Activating Polypeptide Depolarization of Rat Sympathetic Neurons J. Neurosci., October 1, 2000; 20(19): 7353 - 7361. [Abstract] [Full Text] [PDF] D. Vaudry, B. J. Gonzalez, M. Basille, L. Yon, A. Fournier, and H. Vaudry Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: From Structure to Functions Pharmacol. Rev., June 1, 2000; 52(2): 269 - 324. 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